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Analysis of frankincense essential oil compositions by GC-MS

1 Instruments and reagents
   GC-MS3100 gas chromatograph/mass spectrometer (EAST & WEST ANALYTICAL INSTRUMENTS, INC); Equity-5 (30m×0.25mm×0.25um) silica capillary column; 1 mL micro injector;
Sample: frankincense essential oil
2 Analysis conditions
MS conditions: EI source; ion source temperature: 200℃; electron energy: 70eV; scanning mode：full scan; scanning range: 28.5u～400u; scanning period: 0.6 s; interface temperature: 250 ℃; multiplier high voltage: 1200 V.
GC conditions: Equity-5 (30m×0.25mm×0.25um) silica capillary column; injection port temperature: 250 ℃; split sampling; sample volume: 0.08 mL; column flow: 1 mL/min; split ratio: 70:1; precolumn pressure: 50 kPa; purge rate: 2ml/min; column temperature program: hold at 50℃ for 1min, ramp up to 250 ° C at 5 °C/min and then hold for 10 min.
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Figure 1 TIC chromatogram of frankincense essential oil
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Figure 2 Partially magnified chromatogram
 
 
Table 1 Analysis results of frankincense essential oil composition
Peak No. Retention time, min Name CAS No Molecular formula relative amount, % similarity degree, %
1 7.15 1-Isopropyl-4-methylbicyclo[3.1.0]hex-2-ene 58037-87-9 C10H16 64.41 95
2 7.30 Bicyclo[3.1.1]hept-2-ene, 2,6,6-trimethyl-, (1R)- 7785-70-8 C10H16 5.43 93
3 7.55 Bicyclo[3.1.0]hex-2-ene, 4-methylene-1-(1-methylethyl)-
（2,4(10)-Thujadiene）		 36262-09-6 C10H14 0.21 83
4 7.68 4-Carene, (1S,3R,6R)-(-)- 5208-49-1 C10H16 0.06 74
5 8.40 Phellandrene 555-10-2 C10H16 4.92 90
6 8.50 beta-Pinene 127-91-3 C10H16 0.29 83
7 8.86 1,6-Octadiene, 7-methyl-3-methylene-
（Myrcene）		 123-35-3 C10H16 0.67  
8 9.30 1,3-Cyclohexadiene, 2-methyl-5-(1-methylethyl)- 99-83-2 C10H16 2.14 91
9 9.49 3-Carene 13466-78-9 C10H16 4.14  
10 9.66 Cyclohexene, 1-methyl-4-(1-methylethylidene)- 586-62-9 C10H16 0.21 85
11 9.91 Benzene, 1-methyl-4-(1-methylethyl)-
（p-Cymene）		 99-87-6 C10H14 2.40 90
12 10.05 D-Limonene 5989-27-5 C10H16 2.11 88
13 10.28 1,3,6-Octatriene, 3,7-dimethyl-, (E)- 3779-61-1 C10H16 0.43 91
14 10.60 1,3,6-Octatriene, 3,7-dimethyl-, (Z)- 3338-55-4 C10H16 0.17 81
15 10.95 1,4-Cyclohexadiene, 1-methyl-4-(1-methylethyl)- 99-85-4 C10H16 0.47 85
16 11.26 5-Isopropyl-2-methylbicyclo[3.1.0]hexan-2-ol
cis-Sabinenehydrate		 15826-82-1 C10H18O 0.12 82
17 11.87 2-Carene 554-61-0 C10H16 0.18 86
18 12.22 Terpineol, cis- 7299-41-4 C10H18O 0.20 80
19 12.78 Thujone 546-80-5 C10H16O 0.16 86
20 14.67 1-Methyl-4-isopropyl-1-cyclohexen-4-ol （4-Terpineol） 562-74-3 C10H18O 0.76 84
21 15.28 Anisole, p-allyl- 140-67-0 C10H12O 2.61 92
22 20.55 Tricyclo[4.4.0.0(2,7)]dec-3-ene, 8-isopropyl-1,3-dimethyl- 3856-25-5 C15H24 0.10 74
23 20.82 Cyclobuta[1,2:3,4]dicyclopentene, decahydro-3a-methyl-6-methylene-1-(1-methylethyl)-, 5208-59-3 C15H24 0.46 88
24 21.19 Benzene, 1,2-dimethoxy-4-(2-propenyl)- 93-15-2 C11H14O2 0.26 84
25 21.26 Humulen-(v1) N/A C15H24 0.03 74
26 22.04 Germacrene D 23986-74-5 C15H24 0.04 76
27 22.12 Bicyclo[3.1.1]hept-2-ene, 2,6-dimethyl-6-(4-methyl-3-pentenyl)- 17699-05-7 C15H24 0.04 78
28 23.43 Naphthalene, 1,2,4a,5,6,8a-hexahydro-4,7-dimethyl-1-(1-methylethyl)- 483-75-0 C15H24 0.04 80
29 23.50 Isocaryophillene N/A C15H24 0.05 81
30 24.48 Cadina-1(10),4-diene 483-76-1 C15H24 0.09 70
31 24.68 VERIDIFLOROL N/A C15H26O 0.55 68
32 25.17 Benzene, 1,2,3-trimethoxy-5-(2-propenyl)- 487-11-6 C12H16O3 0.07 81
33 31.03 Bicyclo[3.1.0]hex-2-ene, 2-methyl-5-(1-methylethyl)-
（3-Thujene）		 2867-05-2 C10H16 0.05 76
34 31.50 Isopropyl Myristate 110-27-0 C17H34O2 6.14 88
 
 

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